Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293)

Rajaa Umran; Abbas Mol; Amal Umran Mosa; Murat Kaya

doi:10.29002/asujse.1382667

EN

Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293)

Abstract

Intact chitosan film was produced from Bradyporus sp. (Orthoptera, Insecta) and characterized by Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM) and elemental analysis. MTT test was performed to determine the cytotoxic effects of chitosan. Chitosan administered at optimal and overdose intervals was applied to HEK293 cells for 24, 48 and 72 hours. In the MTT cytotoxicity test, the IC 50 dose of chitosan was 1427 µM at 24 hours, 808.9 µM at 48 hours, and 611.6 µM at 72 hours. Considering all the conducted analyses and cytotoxic experiments, the obtained intact novel chitosan film from the insects could find applications for biotechnological applications.

Keywords

References

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Details

Primary Language

English

Subjects

Biomaterial

Journal Section

Research Article

Authors

Rajaa Umran
0000-0003-1716-5509
Türkiye

Abbas Mol
0000-0003-0660-967X
Türkiye

Amal Umran Mosa
0000-0003-0395-1509
Iraq

Murat Kaya ^*
0000-0001-6954-2703
Türkiye

Publication Date

June 30, 2024

Submission Date

October 28, 2023

Acceptance Date

December 11, 2023

Published in Issue

Year 1970 Volume: 8 Number: 1

DOI

https://doi.org/10.29002/asujse.1382667

IZ

https://izlik.org/JA45CR24GH

Cite

RIS / Bibtex

APA

Umran, R., Mol, A., Mosa, A. U., & Kaya, M. (2024). Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293). Aksaray University Journal of Science and Engineering, 8(1), 16-23. https://doi.org/10.29002/asujse.1382667

Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293)

Abstract

Keywords

Öz

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite