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Determination of The Potential Using in The Treatment of Common Chronic Diseases of Anchusa Azurea Kurdica Roots Different Extract

Yıl 2024, , 1 - 15, 30.06.2024

Ako Hamasaeed Abdulqadir , İsmail Şen , Gokhan Zengin , Yavuz Selim Cakmak

https://doi.org/10.29002/asujse.1369488

Öz

Today, some common chronic diseases still have no known treatment. For this reason, studies on method and drug development for the treatment of these diseases continue. Even if synthetic drugs are used successful in treatment, it is becoming increasingly common to prefer natural products in these drug development studies due to they have some side effects. This study focused on determining the antioxidant and enzyme inhibition activities of Anchusa azurea var. kurdica roots and revealing the plant's potential to be used in the treatment of diseases such as diabetes, Alzheimer's and cancer. Herein, antioxidant activities of extracts obtained from root parts with different solvents were determined by free radical scavenging activity (DPPH, ABTS), FRAP, CUPRAC, phosphomolybdate and metal chelation activity tests. Enzyme inhibition activities were investigated for cholinesterase (acetyl and butyryl), α-amylase, α-glucosidase and tyrosinase enzymes. In addition, the total phenolic and flavonoid contents of the extracts were determined as well as their phenolic compounds. In antioxidant activity tests, methanol extract generally showed higher activity compared the others, while ethyl acetate extract was found to be more active in enzyme inhibition. While the total phenolic content in the methanol extract was found at 22.12 mg GAE/g extract, the flavonoid content was found at very low levels in all extracts. Quercetin and rosmarinic acid were high amounts components in the phenolic compositions of the extracts. According to the obtained datas, A. azurea var. kurdica root extracts showed a good antioxidant and enzyme inhibition activities. The results showed that the plant has a high potential to be used as raw material for drug development studies for the treatment of chronic diseases.

Anahtar Kelimeler

Bioactivity Traditional medicine Phytochemistry Chronic diseases Natural compounds

Teşekkür

Bu çalışma doktora bir kısmını içermektedir. Çalışmada kullanılan bitkilerin teşhisinde destek aldığımız Selçuk Üniversitesi Fen Fakültesi Biyoteknoloji Bölümü Öğretim Üyesi Prof. Dr. Evren YILDIZTUGAY’a teşekkür ederiz.

Kaynakça

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Anchusa Azurea var. Kurdica Köklerinin Farklı Özütlerinin Yaygın Kronik Hastalıkların Tedavisinde Kullanım Potansiyellerinin Belirlenmesi

Yıl 2024, , 1 - 15, 30.06.2024

Ako Hamasaeed Abdulqadir , İsmail Şen , Gokhan Zengin , Yavuz Selim Cakmak

https://doi.org/10.29002/asujse.1369488

Öz

Günümüzde yaygın olarak görülen bazı kronik hastalıkların hala bilinen bir tedavisi yoktur. Bu nedenle bu hastalıkların tedavisi için yöntem ve ilaç geliştirme çalışmaları devam etmektedir. Sentetik ilaçlar tedavide başarılı bir şekilde kullanılsa bile, bazı yan etkileri nedeniyle bu ilaç geliştirme çalışmalarında doğal ürünlerin tercih edilmesi giderek yaygınlaşmaktadır. Bu çalışma Anchusa azurea var. kurdica köklerinin antioksidan ve enzim inhibisyon aktivitelerinin belirlenerek bitkinin diyabet, Alzheimer ve kanser gibi hastalıkların tedavisinde kullanılabilme potansiyelinin ortaya konulmasına odaklanmıştır. Burada kök kısımlarından farklı çözücüler ile elde edilen özütlerinin antioksidan aktiviteleri serbest radikal giderme aktivitesi (DPPH, ABTS), FRAP, CUPRAC, fosfomolibdat ve metal şelatlama aktivite testleri ile belirlenmiştir. Enzim inhibisyon aktiviteleri kolinesteraz (asetil ve bütiril), α-amilaz, α-glukozidaz ve tirozinaz enzimleri için araştırılmıştır. Ayrıca özütlerin toplam fenolik ve flavonoid içerikleri yanında fenolik bileşimleri de belirlenmiştir. Antioksidan aktivite testlerinde genel olarak metanol özütü diğerlerine kıyasla yüksek aktivite gösterirken, enzim inhibisyonunda etil asetat özütü daha aktif bulunmuştur. Metanol ekstraktındaki toplam fenolik içerik 22,12 mg GAE/g ekstrakt olarak bulunurken, flavonoid içeriği tüm ekstraktlarda oldukça düşük seviyelerde bulunmuştur. Özütlerin fenolik bileşimlerinde kuersetin ve rosmarinik asit yüksek oranda bulunan fenolik bileşenler olmuştur. Elde edilen verilere göre A. azurea var. kurdica kök özütleri iyi düzeyde antioksidan ve enzim inhibisyon aktivitesi göstermiştir. Sonuçlar bitkinin kronik hastalıklarının tedavisine yönelik ilaç geliştirme çalışmalarında hammadde olarak kullanım potansiyelinin yüksek olduğunu göstermiştir.

Anahtar Kelimeler

Biyoaktivite Geleneksel tıp Fitokimya Kronik hastalıklar Doğal bileşenler

Kaynakça

  • [1] Aktumsek, A., Zengin, G., Guler, G. O., Cakmak, Y. S., ve Duran, A. (2013). Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species, Food and Chemical Toxicology, 55, 290-296.
  • [2] Xu, X., Liu, A., Hu, S., Ares, I., Martínez-Larrañaga, M. R., Wang, X., ve Martínez, M. A. (2021). Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action, Food Chemistry, 353, 129488.
  • [3] Pham-Huy, L. A., He, H. ve Pham-Huy, C. (2008). Free radicals, antioxidants in disease and health, International Journal of Biomedical Science, 4, 2, 89-96.
  • [4] Di Meo, S., ve Venditti, P. (2020). Evolution of the knowledge of free radicals and other oxidants, Oxidative Medicine and Cellular Longevity, 2020, 1-32.
  • [5] Rekatsina, M., Paladini, A., Piroli, A., Zis, P., Pergolizzi, J. V. ve Varrassi, G. (2020). Pathophysiology and therapeutic perspectives of oxidative stress and neurodegenerative diseases: a narrative review, Advances in Therapy, 37, 1, 113-139.
  • [6] Akbari, B., Baghaei‐Yazdi, N., Bahmaie, M., ve Mahdavi Abhari, F. (2022). The role of plant‐derived natural antioxidants in reduction of oxidative stress, BioFactors, 48, 3, 611-633.
  • [7] Lopa, S. S., Al-Amin, M., Hasan, M., Ahammed, M., Islam, K. M., Alam, A. H. M. ve Sadik, M. (2021). Phytochemical analysis and cholinesterase inhibitory and antioxidant activities of Enhydra fluctuans relevant in the management of Alzheimer’s disease, International Journal of Food Science, 8862025.
  • [8] Li, Y., Zhang, W., Zhao, R. ve Zhang, X. (2022). Advances in oral peptide drug nanoparticles for diabetes mellitus treatment, Bioactive Materials, 15, 392-408.
  • [9] Robinson, P. K. (2015). Enzymes: principles and biotechnological applications, Essays in Biochemistry, 59, 1-41.
  • [10] Isah, T. (2019). Stress and defense responses in plant secondary metabolites production, Biological Research, 52.
  • [11] Tiwari, R., ve Rana, C. S. (2015). Plant secondary metabolites: a review, International Journal of Engineering Research and General Science, 3, 5, 661-670.
  • [12] Hussain, F. H. S., Ahamad, J., ve Osw, P. S. (2019). A Comprehensive Review on Pharmacognostical and Pharmacological Characters of Anchusa Azurea, Advances in Medical, Dental and Health Sciences, 3, 33-37.
  • [13] Petersen, M., & Simmonds, M. S. (2003). Rosmarinic acid, Phytochemistry, 62, 2, 121-125.
  • [14] Lau, C. H., Chua, L. S., Lee, C. T., & Aziz, R. (2015). Fractionation of rosmarinic acid from crude extract of Orthosiphon stamineus by solid phase extraction. Journal of Engineering Science and Technology, 10, 104-112.
  • [15] Amoah, S. K., Sandjo, L. P., Kratz, J. M., & Biavatti, M. W. (2016). Rosmarinic acid–pharmaceutical and clinical aspects. Planta Medica, 82, 05, 388-406.
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Toplam 71 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Enzimler
Bölüm Araştırma Makalesi
Yazarlar

Ako Hamasaeed Abdulqadir 0000-0001-5404-0938

İsmail Şen 0000-0002-1285-659X

Gokhan Zengin 0000-0001-6548-7823

Yavuz Selim Cakmak 0000-0001-8954-5485

Yayımlanma Tarihi 30 Haziran 2024
Gönderilme Tarihi 2 Ekim 2023
Kabul Tarihi 26 Ekim 2023
Yayımlandığı Sayı Yıl 2024

Kaynak Göster

APA Abdulqadir, A. H., Şen, İ., Zengin, G., Cakmak, Y. S. (2024). Anchusa Azurea var. Kurdica Köklerinin Farklı Özütlerinin Yaygın Kronik Hastalıkların Tedavisinde Kullanım Potansiyellerinin Belirlenmesi. Aksaray University Journal of Science and Engineering, 8(1), 1-15. https://doi.org/10.29002/asujse.1369488
Aksaray J. Sci. Eng. | e-ISSN: 2587-1277 | Period: Biannually | Founded: 2017 | Publisher: Aksaray University | https://asujse.aksaray.edu.tr