Anchusa Azurea var. Kurdica Köklerinin Farklı Özütlerinin Yaygın Kronik Hastalıkların Tedavisinde Kullanım Potansiyellerinin Belirlenmesi

Ako Hamasaeed Abdulqadir; İsmail Şen; Gokhan Zengin; Yavuz Selim Cakmak

doi:10.29002/asujse.1369488

TR EN

Anchusa Azurea var. Kurdica Köklerinin Farklı Özütlerinin Yaygın Kronik Hastalıkların Tedavisinde Kullanım Potansiyellerinin Belirlenmesi

Öz

Günümüzde yaygın olarak görülen bazı kronik hastalıkların hala bilinen bir tedavisi yoktur. Bu nedenle bu hastalıkların tedavisi için yöntem ve ilaç geliştirme çalışmaları devam etmektedir. Sentetik ilaçlar tedavide başarılı bir şekilde kullanılsa bile, bazı yan etkileri nedeniyle bu ilaç geliştirme çalışmalarında doğal ürünlerin tercih edilmesi giderek yaygınlaşmaktadır. Bu çalışma Anchusa azurea var. kurdica köklerinin antioksidan ve enzim inhibisyon aktivitelerinin belirlenerek bitkinin diyabet, Alzheimer ve kanser gibi hastalıkların tedavisinde kullanılabilme potansiyelinin ortaya konulmasına odaklanmıştır. Burada kök kısımlarından farklı çözücüler ile elde edilen özütlerinin antioksidan aktiviteleri serbest radikal giderme aktivitesi (DPPH, ABTS), FRAP, CUPRAC, fosfomolibdat ve metal şelatlama aktivite testleri ile belirlenmiştir. Enzim inhibisyon aktiviteleri kolinesteraz (asetil ve bütiril), α-amilaz, α-glukozidaz ve tirozinaz enzimleri için araştırılmıştır. Ayrıca özütlerin toplam fenolik ve flavonoid içerikleri yanında fenolik bileşimleri de belirlenmiştir. Antioksidan aktivite testlerinde genel olarak metanol özütü diğerlerine kıyasla yüksek aktivite gösterirken, enzim inhibisyonunda etil asetat özütü daha aktif bulunmuştur. Metanol ekstraktındaki toplam fenolik içerik 22,12 mg GAE/g ekstrakt olarak bulunurken, flavonoid içeriği tüm ekstraktlarda oldukça düşük seviyelerde bulunmuştur. Özütlerin fenolik bileşimlerinde kuersetin ve rosmarinik asit yüksek oranda bulunan fenolik bileşenler olmuştur. Elde edilen verilere göre A. azurea var. kurdica kök özütleri iyi düzeyde antioksidan ve enzim inhibisyon aktivitesi göstermiştir. Sonuçlar bitkinin kronik hastalıklarının tedavisine yönelik ilaç geliştirme çalışmalarında hammadde olarak kullanım potansiyelinin yüksek olduğunu göstermiştir.

Anahtar Kelimeler

Determination of The Potential Using in The Treatment of Common Chronic Diseases of Anchusa Azurea Kurdica Roots Different Extract

Abstract

Today, some common chronic diseases still have no known treatment. For this reason, studies on method and drug development for the treatment of these diseases continue. Even if synthetic drugs are used successful in treatment, it is becoming increasingly common to prefer natural products in these drug development studies due to they have some side effects. This study focused on determining the antioxidant and enzyme inhibition activities of Anchusa azurea var. kurdica roots and revealing the plant's potential to be used in the treatment of diseases such as diabetes, Alzheimer's and cancer. Herein, antioxidant activities of extracts obtained from root parts with different solvents were determined by free radical scavenging activity (DPPH, ABTS), FRAP, CUPRAC, phosphomolybdate and metal chelation activity tests. Enzyme inhibition activities were investigated for cholinesterase (acetyl and butyryl), α-amylase, α-glucosidase and tyrosinase enzymes. In addition, the total phenolic and flavonoid contents of the extracts were determined as well as their phenolic compounds. In antioxidant activity tests, methanol extract generally showed higher activity compared the others, while ethyl acetate extract was found to be more active in enzyme inhibition. While the total phenolic content in the methanol extract was found at 22.12 mg GAE/g extract, the flavonoid content was found at very low levels in all extracts. Quercetin and rosmarinic acid were high amounts components in the phenolic compositions of the extracts. According to the obtained datas, A. azurea var. kurdica root extracts showed a good antioxidant and enzyme inhibition activities. The results showed that the plant has a high potential to be used as raw material for drug development studies for the treatment of chronic diseases.

Keywords

Teşekkür

Bu çalışma doktora bir kısmını içermektedir. Çalışmada kullanılan bitkilerin teşhisinde destek aldığımız Selçuk Üniversitesi Fen Fakültesi Biyoteknoloji Bölümü Öğretim Üyesi Prof. Dr. Evren YILDIZTUGAY’a teşekkür ederiz.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Enzimler

Bölüm

Araştırma Makalesi

Yazarlar

Ako Hamasaeed Abdulqadir ^*
0000-0001-5404-0938
Türkiye

İsmail Şen
0000-0002-1285-659X
Türkiye

Gokhan Zengin
0000-0001-6548-7823
Türkiye

Yavuz Selim Cakmak
0000-0001-8954-5485
Türkiye

Yayımlanma Tarihi

30 Haziran 2024

Gönderilme Tarihi

2 Ekim 2023

Kabul Tarihi

26 Ekim 2023

Yayımlandığı Sayı

Yıl 1970 Cilt: 8 Sayı: 1

DOI

https://doi.org/10.29002/asujse.1369488

IZ

https://izlik.org/JA55SZ57LN

Kaynak Göster

RIS / Bibtex

APA

Abdulqadir, A. H., Şen, İ., Zengin, G., & Cakmak, Y. S. (2024). Anchusa Azurea var. Kurdica Köklerinin Farklı Özütlerinin Yaygın Kronik Hastalıkların Tedavisinde Kullanım Potansiyellerinin Belirlenmesi. Aksaray University Journal of Science and Engineering, 8(1), 1-15. https://doi.org/10.29002/asujse.1369488