Atık Yılan Gömleğinin Taşıyıcı Sistem Olarak Değerlendirilmesi ve D-Panthenol’ün in Vitro Salımı

Betül Aktaş; Lalehan Akyüz; Murat Kaya

doi:10.29002/asujse.1298383

EN TR

Evaluation of Waste Snake Molt as a Delivery System and in Vitro Release of D-Panthenol

Abstract

Biomolecules such as collagen, silk and keratin are frequently preferred in medical fields as wound healing and drug delivery systems, thanks to their many unique properties. Keratin-based materials, which are a strong structural proteins such as feathers, hooves, hair and wool, are preferred for drug release because they are nanotoxic and biocompatible. However, no studies have been conducted on the drug release of the snake skin containing keratin as a structural protein. Controlled drug release is very important in terms of maximum benefit, minimum harm and patient comfort from therapeutic agents. In the present study, the waste shedding of Dolichophis caspius (Hazer snake, bozyoruk) which has high keratin content and widely distributed was evaluated as a drug carrier biomaterial for the first time. D-Panthenol, which is commonly used in wound healing, was loaded on three different parts of the untreated waste snake skin, dorsal scale, ventral scale and hinge, and its release properties were investigated. Average drug loading capacities for the dorsal scale, ventral scale and hinge portions, were recorded as 2.07±0.13%, 1.28%±0.04% and 1.77±0.95%. The release was measured by taking sample for every hour at least for 24 hours. In the first hour, 46.67% of the drug was released in the dorsal scale, 80.09% in the ventral scale and 69.85% in the hinge portion. Based on these results, especially the dorsal scale part was developed in future studies and it was revealed that it is a potential biomaterial for controlled drug release.

Keywords

Atık Yılan Gömleğinin Taşıyıcı Sistem Olarak Değerlendirilmesi ve D-Panthenol’ün in Vitro Salımı

Öz

Kolajen, ipek ve keratin gibi biyomoleküller sahip oldukları birçok eşsiz özellikleri sayesinde yara iyileştirme ve ilaç taşıma sistemleri olarak medikal alanda sıkça tercih edilmektedir. Özellikle tüy, toynak, saç ve yünde bulunan, güçlü bir yapısal protein olan keratin bazlı materyaller, nantoksik ve biyouyumlu olmalarından dolayı ilaç salımı amaçlı tercih edilmektedir. Fakat yapısal protein olarak keratin içeren yılan gömleği için ilaç salımı üzerine herhangi bir çalışma yapılmamıştır. Kontrollü ilaç salımı, terapötik ajanlardan maksimum fayda sağlanması, minimum zarar görülmesi ve hasta konforu açısından oldukça önemlidir. Mevcut çalışmada, yüksek keratin içeriğine sahip ve oldukça geniş bir yayılım gösteren Dolichophis caspius’ un (Hazer yılanı, bozyörük) atık gömleği ilk kez ilaç taşıyıcı biyomalzeme olarak değerlendirilmiştir. Hiçbir işlem görmemiş atık yılan derisinin dorsal ölçek, ventral ölçek ve menteşe olmak üzere üç farklı kısmına, yara iyileşmesinde yaygın olarak kullanılan D-Panthenol yüklenmiş ve salım özellikleri araştırılmıştır. Sırasıyla dorsal ölçek, ventral ölçek ve menteşe kısımları için ortalama ilaç yükleme kapasiteleri: %2,07±0,13, %1,28±0,04, %1,77±0,95 olarak kaydedilmiştir. Salım 24 saat boyunca her saat başı örnek alınıp ölçüm yapılarak izlenmiştir. İlk 1 saatte dorsal ölçekte ilacın %46.67’si, ventral ölçekte %80.09’u ve menteşe kısmında %69.85’i salınmıştır. Bu sonuçlara dayanarak özellikle dorsal ölçek kısmının ileriki çalışmalarda geliştirilerek kontrollü ilaç salımı için potansiyel bir biyomalzeme olduğu ortaya konulmuştur.

Anahtar Kelimeler

Thanks

Yazarlar, Aksaray Üniversitesi Bilimsel ve Teknolojik Uygulama ve Araştırma Merkezi tarafından sağlanan teknik desteğe teşekkür eder. Araştırma görevlisi Bahar Akyüz Yılmaz’a yardımından dolayı teşekkür eder.

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Details

Primary Language

Turkish

Subjects

Synthetic Biology

Journal Section

Research Article

Authors

Betül Aktaş
0000-0001-9235-7178
Türkiye

Lalehan Akyüz
0000-0001-8548-3037
Türkiye

Murat Kaya ^*
0000-0001-6954-2703
Türkiye

Publication Date

December 30, 2023

Submission Date

May 17, 2023

Acceptance Date

July 2, 2023

Published in Issue

Year 2023 Volume: 7 Number: 2

DOI

https://doi.org/10.29002/asujse.1298383

IZ

https://izlik.org/JA68KH43NP

Cite

RIS / Bibtex

APA

Aktaş, B., Akyüz, L., & Kaya, M. (2023). Atık Yılan Gömleğinin Taşıyıcı Sistem Olarak Değerlendirilmesi ve D-Panthenol’ün in Vitro Salımı. Aksaray University Journal of Science and Engineering, 7(2), 99-106. https://doi.org/10.29002/asujse.1298383

Cited By

Green Modified Graphene Oxide Nanoplatelets as Drug Adsorbents

Journal of Research in Pharmacy

https://doi.org/10.12991/jrespharm.1718624