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Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation

Yıl 2015, Cilt: 21 Sayı: 7, 314 - 318, 02.01.2016

Öz

This study concerns with the combinations of soybean meal (SM), wheat bran (WB) and whey were utilized as the substrates for α-amylase production by Bacillus amyloliquefaciens NRRL B-645. As a result of the experiments, the highest α-amylase activity (4257 U/ml) was obtained containing SM 20 g/l, WB 5 g/l, whey 5% (v/v), peptone 1 g/l, yeast extract (YE) 0.5 g/l and (NH4)2SO4 1 g/l at 33 °C and 150 rpm for 48 h. This innovative process for the α-amylase production can be extended to different enzymes using various agro-industrial wastes.

Kaynakça

  • Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman, G. “Enzymatic Properties of a Novel Thermostable, Thermophilic, Alkaline and Chelator Resistant Amylase From an Alkaliphilic Bacillus sp Isolate ANT-6”. Process Biochemistry, 38(10), 1397-1403, 2003.
  • Torrado A, Vazquez J, Prieto M, Fucinos P, Montemayor M, Pastrana L, Gonzalez M, Murado M. “Amylase Production by Aspergillus oryzae in a Solid-State Bioreactor with Fed-Batch Operation using Mussel Processing Waste Waters as Feeding Medium”. Journal of Chemical Technology and Biotechnology, 88(2), 226–236. 2013.
  • Bessler C, Schmitt J, Maurer KH, Schmid RD. “Directed Evolution of a Bacterial α-amylase: Toward Enhanced pH-Performance and Higher Specific Activity”. Protein Science, 12(10), 2141-2149, 2003.
  • Rodriguez CS, Rodriguez R, Gallego PP, Sanroman A. “Biodegradation of Grape Cluster Stems and Ligninolytic Enzyme Production by Phanerochaete Chrysosporium during Semi-Solid-State Cultivation”. Acta Biotechnologica, 23(1), 65-74, 2003.
  • Economou CN, Makri A, Aggelis G, Pavlou S, Vayenas DV. “Semi-Solid State Fermentation of Sweet Sorghum for the Biotechnological Production of Single Cell Oil”. Bioresource Technology, 101(4), 1385-1388, 2010.
  • Naveena BJ, Altaf M, Bhadrayya K, Reddy C. “Production of L(+) Lactic Acid by Lactobacillus amylophilus GV6 in SemiSolid State Fermentation using Wheat Bran”. Food Technology and Biotechnology, 42(3), 147-152, 2004.
  • Gema H, Kavadia A, Dimou D, Tsagou V, Komaitis M, Aggelis G. “Production of Gamma-Linolenic Acid by Cunninghamella Echinulata Cultivated on Glucose and Orange Peel”. Applied Microbiology Biotechnology, 58(3), 303-307, 2002.
  • Krishna C, Chandrasekaran M. “Banana Waste as Substrate for α-amylase Production by Bacillus subtilis (CBTK 106) under Solid State Fermentation”. Applied Microbiology Biotechnology, 46(2), 106-111, 1996.
  • Xu H, Sun LP, Zhao DQ, Zhang B, Shi YZ, Wu YH. “Production of α-amylase by Aspergillus oryzae as 3951 in Solid State Fermentation using Spent Brewing Grains as Substrate”. Journal of the Sciences Food and Agriculture, 88(3), 529-535, 2008.
  • Murthy PS, Naidu MM, Srinivas P. “Production of α-amylase Under Solid-State Fermentation Utilizing Coffee Waste”. Journal Chemical Technology and Biotechnology, 84(8), 1246-1249, 2009.
  • Erdal S, Taskin M. “Production of α-amylase by Penicillium Expansum MT-1 in Solid-State Fermentation using Waste Loquat (Eriobotrya Japonica Lindley) Kernels as Substrate”. Romanian Biotechnological Letters 15(3), 5342-5350, 2010.
  • Chutmanop J, Chuichulcherm S, Chisti Y, Sirinophakun P. “Protease Production by Aspergillus oryzae in Solid-State Fermentation using Agroindustrial Substrates”. Journal Chemical Technology and Biotechnology, 83(7), 1012-1018, 2008.
  • Hashemi M, Razavi SH, Shojaosadati SA, Mousavi SM, Khajeh K, Safari M. “Development of a Solid-State Fermentation Process for Production of an Alpha Amylase with Potentially Interesting Properties”. Journal of Bioscience and Bioengineering, 110(3), 333-337, 2010.
  • Karatas H, Uyar F, Tolan V, Baysal Z. “Optimization and Enhanced Production of α-amylase and Protease by a Newly Isolated Bacillus licheniformis ZB-05 under Solid-State Fermentation”. Annals of Microbiology, 63(1), 45-52, 2013.
  • Sukumprasertsri M, Unrean P, Pimsamarn J, Kitsubun P, Tongta A. “Fuzzy Logic Control of Rotating Drum Bioreactor for Improved Production of Amylase and Protease Enzymes by Aspergillus oryzae in Solid-State Fermentation”. Journal of Microbiology and Biotechnology, 23(3), 335-342, 2013.
  • Anderson E, Johanson AC, Hogerdal BH. “α-amylase Production in Aqueous two Phase Systems with Bacillus subtilis”. Enzyme and Microbial Technology, 7(7), 333-338, 1985.
  • Tanyildizi MS, Özer D, Elibol M. “Optimization of α-amylase Production by Bacillus sp. using Response Surface Methodology”. Process Biochemistry, 40(7), 2291-2296, 2005.
  • Boesel LF, Azevedo HS, Reis RL. “Incorporation of Alpha-amylase Enzyme and a Bioactive Filler into Hydrophilic, Partially Degradable and Bioactive Cements (HDBCs) as a New Approach to Tailor Simultaneously Their Degradation and Bioactive Behavior”. Biomacromolecules, 7(9), 2600-2609, 2006.
  • Pedersen H, Nielsen J. “The Influence of Nitrogen Sources on the α-amylase Productivity of Aspergillus oryzae in Continuous Cultures”. Applied Microbiology. Biotechnology, 53(3), 278-281, 2000.
  • Pandey A. “Improvement in Solid-State Fermentation for Glucoamylase Production”. Biological Wastes, 34(1), 11-19, 1990.
  • Sodhi HK, Sharma K, Gupta JK, Soni SK. “Production of a Thermostable α-amylase from Bacillus sp. PS-7 by Solid State Fermentation and its Synergistic use in the hydrolysis of Malt Starch for Alcohol Production”. Process Biochemistry, 40(2), 525-534, 2005.
  • Tanyildizi MS, Özer D, Elibol M. “Production of Bacterial α-amylase by B. amyloliquefaciens under Solid Substrate Fermentation”. Biochemical Engineering Journal, 37(3), 294-297, 2007.
  • Syu MJ, Chen YH. “A Study on the α-amylase Fermentation Performed by Bacillus amyloliquefaciens”. Chemical Engineering Journal, 65(3), 237-247, 1997.
  • Akcan N, Serin B, Uyar F. “Production and Optimization Parameters of Amylases from Bacillus subtilis RSKK96 under Solid State Fermentation”. Chemical and Biochemical Engineering Quarterly, 26(3), 233-239, 2012.
  • Anto H, Trivedi U, Patel K. “α-amylase Production by Bacillus cereus MTCC 1305 using Solid-State Fermentation”. Food Technology Biotechnology, 44(2) 241-245, 2006.

Yarı Katı Hal Fermentasyonunda Bacillus amyloliquefaciens ile Alfa Amilaz Üretimine Tarımsal Atıkların Sinerjik Etkileri

Yıl 2015, Cilt: 21 Sayı: 7, 314 - 318, 02.01.2016

Öz

Bu çalışma; Bacillus amyloliquefaciens NRRL B-645 ile substrat olarak soya küspesi (SM), buğday kepeği (WB) ve peynir altı suyu kombinasyonları kullanılarak α-amilaz üretimi ile ilgilidir. Deneyler sonucunda en yüksek enzim aktivitesi SM 20g/l, WB 5g/l, peynir altı suyu 5% (v/v), peptone 1 g/l, yeast ekstrakt (YE) 0.5 g/l ve (NH4)2SO4 1 g/l konsantrasyonunda 48 saat sonunda, 33 °C fermantasyon sıcaklığı, 150 devir/dk karıştırma hızında 4257 U/ml olarak elde edildi. α-amilaz üretimi için kullanılan bu yeni proses, farklı tarımsal atıklar kullanılarak farklı enzimlerin üretiminde de kullanılabilir.

Kaynakça

  • Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman, G. “Enzymatic Properties of a Novel Thermostable, Thermophilic, Alkaline and Chelator Resistant Amylase From an Alkaliphilic Bacillus sp Isolate ANT-6”. Process Biochemistry, 38(10), 1397-1403, 2003.
  • Torrado A, Vazquez J, Prieto M, Fucinos P, Montemayor M, Pastrana L, Gonzalez M, Murado M. “Amylase Production by Aspergillus oryzae in a Solid-State Bioreactor with Fed-Batch Operation using Mussel Processing Waste Waters as Feeding Medium”. Journal of Chemical Technology and Biotechnology, 88(2), 226–236. 2013.
  • Bessler C, Schmitt J, Maurer KH, Schmid RD. “Directed Evolution of a Bacterial α-amylase: Toward Enhanced pH-Performance and Higher Specific Activity”. Protein Science, 12(10), 2141-2149, 2003.
  • Rodriguez CS, Rodriguez R, Gallego PP, Sanroman A. “Biodegradation of Grape Cluster Stems and Ligninolytic Enzyme Production by Phanerochaete Chrysosporium during Semi-Solid-State Cultivation”. Acta Biotechnologica, 23(1), 65-74, 2003.
  • Economou CN, Makri A, Aggelis G, Pavlou S, Vayenas DV. “Semi-Solid State Fermentation of Sweet Sorghum for the Biotechnological Production of Single Cell Oil”. Bioresource Technology, 101(4), 1385-1388, 2010.
  • Naveena BJ, Altaf M, Bhadrayya K, Reddy C. “Production of L(+) Lactic Acid by Lactobacillus amylophilus GV6 in SemiSolid State Fermentation using Wheat Bran”. Food Technology and Biotechnology, 42(3), 147-152, 2004.
  • Gema H, Kavadia A, Dimou D, Tsagou V, Komaitis M, Aggelis G. “Production of Gamma-Linolenic Acid by Cunninghamella Echinulata Cultivated on Glucose and Orange Peel”. Applied Microbiology Biotechnology, 58(3), 303-307, 2002.
  • Krishna C, Chandrasekaran M. “Banana Waste as Substrate for α-amylase Production by Bacillus subtilis (CBTK 106) under Solid State Fermentation”. Applied Microbiology Biotechnology, 46(2), 106-111, 1996.
  • Xu H, Sun LP, Zhao DQ, Zhang B, Shi YZ, Wu YH. “Production of α-amylase by Aspergillus oryzae as 3951 in Solid State Fermentation using Spent Brewing Grains as Substrate”. Journal of the Sciences Food and Agriculture, 88(3), 529-535, 2008.
  • Murthy PS, Naidu MM, Srinivas P. “Production of α-amylase Under Solid-State Fermentation Utilizing Coffee Waste”. Journal Chemical Technology and Biotechnology, 84(8), 1246-1249, 2009.
  • Erdal S, Taskin M. “Production of α-amylase by Penicillium Expansum MT-1 in Solid-State Fermentation using Waste Loquat (Eriobotrya Japonica Lindley) Kernels as Substrate”. Romanian Biotechnological Letters 15(3), 5342-5350, 2010.
  • Chutmanop J, Chuichulcherm S, Chisti Y, Sirinophakun P. “Protease Production by Aspergillus oryzae in Solid-State Fermentation using Agroindustrial Substrates”. Journal Chemical Technology and Biotechnology, 83(7), 1012-1018, 2008.
  • Hashemi M, Razavi SH, Shojaosadati SA, Mousavi SM, Khajeh K, Safari M. “Development of a Solid-State Fermentation Process for Production of an Alpha Amylase with Potentially Interesting Properties”. Journal of Bioscience and Bioengineering, 110(3), 333-337, 2010.
  • Karatas H, Uyar F, Tolan V, Baysal Z. “Optimization and Enhanced Production of α-amylase and Protease by a Newly Isolated Bacillus licheniformis ZB-05 under Solid-State Fermentation”. Annals of Microbiology, 63(1), 45-52, 2013.
  • Sukumprasertsri M, Unrean P, Pimsamarn J, Kitsubun P, Tongta A. “Fuzzy Logic Control of Rotating Drum Bioreactor for Improved Production of Amylase and Protease Enzymes by Aspergillus oryzae in Solid-State Fermentation”. Journal of Microbiology and Biotechnology, 23(3), 335-342, 2013.
  • Anderson E, Johanson AC, Hogerdal BH. “α-amylase Production in Aqueous two Phase Systems with Bacillus subtilis”. Enzyme and Microbial Technology, 7(7), 333-338, 1985.
  • Tanyildizi MS, Özer D, Elibol M. “Optimization of α-amylase Production by Bacillus sp. using Response Surface Methodology”. Process Biochemistry, 40(7), 2291-2296, 2005.
  • Boesel LF, Azevedo HS, Reis RL. “Incorporation of Alpha-amylase Enzyme and a Bioactive Filler into Hydrophilic, Partially Degradable and Bioactive Cements (HDBCs) as a New Approach to Tailor Simultaneously Their Degradation and Bioactive Behavior”. Biomacromolecules, 7(9), 2600-2609, 2006.
  • Pedersen H, Nielsen J. “The Influence of Nitrogen Sources on the α-amylase Productivity of Aspergillus oryzae in Continuous Cultures”. Applied Microbiology. Biotechnology, 53(3), 278-281, 2000.
  • Pandey A. “Improvement in Solid-State Fermentation for Glucoamylase Production”. Biological Wastes, 34(1), 11-19, 1990.
  • Sodhi HK, Sharma K, Gupta JK, Soni SK. “Production of a Thermostable α-amylase from Bacillus sp. PS-7 by Solid State Fermentation and its Synergistic use in the hydrolysis of Malt Starch for Alcohol Production”. Process Biochemistry, 40(2), 525-534, 2005.
  • Tanyildizi MS, Özer D, Elibol M. “Production of Bacterial α-amylase by B. amyloliquefaciens under Solid Substrate Fermentation”. Biochemical Engineering Journal, 37(3), 294-297, 2007.
  • Syu MJ, Chen YH. “A Study on the α-amylase Fermentation Performed by Bacillus amyloliquefaciens”. Chemical Engineering Journal, 65(3), 237-247, 1997.
  • Akcan N, Serin B, Uyar F. “Production and Optimization Parameters of Amylases from Bacillus subtilis RSKK96 under Solid State Fermentation”. Chemical and Biochemical Engineering Quarterly, 26(3), 233-239, 2012.
  • Anto H, Trivedi U, Patel K. “α-amylase Production by Bacillus cereus MTCC 1305 using Solid-State Fermentation”. Food Technology Biotechnology, 44(2) 241-245, 2006.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makale
Yazarlar

Ali Yaraş

Veyis Selen

Dursun Özer

Yayımlanma Tarihi 2 Ocak 2016
Yayımlandığı Sayı Yıl 2015 Cilt: 21 Sayı: 7

Kaynak Göster

APA Yaraş, A., Selen, V., & Özer, D. (2016). Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, 21(7), 314-318.
AMA Yaraş A, Selen V, Özer D. Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. Ocak 2016;21(7):314-318.
Chicago Yaraş, Ali, Veyis Selen, ve Dursun Özer. “Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus Amyloliquefaciens in Semi Solid Substrate Fermentation”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 21, sy. 7 (Ocak 2016): 314-18.
EndNote Yaraş A, Selen V, Özer D (01 Ocak 2016) Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 21 7 314–318.
IEEE A. Yaraş, V. Selen, ve D. Özer, “Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation”, Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, c. 21, sy. 7, ss. 314–318, 2016.
ISNAD Yaraş, Ali vd. “Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus Amyloliquefaciens in Semi Solid Substrate Fermentation”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 21/7 (Ocak 2016), 314-318.
JAMA Yaraş A, Selen V, Özer D. Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. 2016;21:314–318.
MLA Yaraş, Ali vd. “Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus Amyloliquefaciens in Semi Solid Substrate Fermentation”. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, c. 21, sy. 7, 2016, ss. 314-8.
Vancouver Yaraş A, Selen V, Özer D. Synergistic Effects of Agro-Industrial Wastes on Alpha Amylase Production by Bacillus amyloliquefaciens in Semi Solid Substrate Fermentation. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi. 2016;21(7):314-8.





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