INVESTIGATION OF SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RATS WERE GIVEN VACCINIUM MYRTILLUS (BLUEBERRY) EXTRACT

Dide Kılıçalp Kılınç; Seda Nur Aksu; Serdal Öğüt

Araştırma Makalesi

INVESTIGATION OF SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RATS WERE GIVEN VACCINIUM MYRTILLUS (BLUEBERRY) EXTRACT

Yıl 2018, Cilt: 2 Sayı: 2, 65 - 72, 02.05.2018

Dide Kılıçalp Kılınç Seda Nur Aksu Serdal Öğüt

Öz

Aim: In this study, the differences in hematological and biochemical parameters
were investigated in rats given Vaccinium myrtillus (blueberry)
extract.

Method: Biochemical
parameters albumin (Alb), alkaline phosphatase (ALP), alanine aminotransferase
(ALT), amylase (AMY), blood urea nitrogen (BUN), calcium (Ca), creatinine
(CRE), potassium (K), sodium (Na), phosphorus (Phos), total bilirubin (TBIL),
total protein (TP), fasting glucose (FG), total cholesterol (TC), and
hematologic parameters such as WBC, LYM, MON, NEU, LY, MO, NE, RBC, HGB, HCT
MCV, MCH, MCHC, RDW, PLT, PCT, MPV, PDW levels were measured. In the study,
rats were used as animal material. The rats were separated into two groups,
each of which included 10 rats. Ad libitum fed rats were subjected to the
control group. In the group of blueberry extract for a period of 8 weeks of the
experiment the rate of 20 mg/kg were mixed with drinking water.

Results: Completion
of the process of the experiment, after obtaining blood samples from the above
parameters, creatinine: 0.48 from 0:41 to fell in value. MPV: 6.48 from 6.30 a,
PDW: 33.40 from the 32.48 level have fallen. But no significant changes were
observed outside of these parameters.

Conclusion: As a result, there is a negative impact on blood parameters of
blueberry on the contrary due to the significant decrease in the values of MPV
and PDW, which is a risk factor for hardening of the arteries as a result of
oxidative damage fragmented platelets sticking to the walls of arteries and
reduce the risk of atherosclerosis be considered.

Anahtar Kelimeler

Biochemical markers, hematological tests, rat

Kaynakça

1. Ercişli S. Chemical composition of fruits in some rose (Rosa spp.) species. Food Chemistry. 2007;104(4):1379-1384.
2. Srividya AR, Nagasamy V, Vishnuvarthan VJ. Nutraceuticals as medicine: a review. Pharmanest. 2010;1(2):132–145.
3. Abuajah CI, Ogbonna AC, Osuji CM. Functional components and medicinal properties of food: a review. Journal Of Food Science And Technology. 2015;52:2522–2529.
4. Alvarez-Suarez JM, Giampieri F, Tulipan S, Casoli T, Stefano G Di, González-Paramás AM et al. One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans. The Journal Of Nutritional Biochemistry. 2014;25:289–294.
5. Wu X, Cao G, Prior RL. Absorption and metabolism of anthocyanins in elderly women after consumption of elderberry or blueberry. The Journal Of Nutrition. 2002;132:1865–1871.
6. Yi WG, Fischer J, Krewer G, Akoh CC. Phenolic compounds from blueberries can inhibit colon cancer cell proliferation and induce apoptosis. Journal Of Agricultural And Food Chemistry. 2005;53:7320–7329.
7. Li H, Deng Z, Zhu H, Hongyan Li, Zhu H, Hu C. et al. Highly pigmented vegetables: Anthocyanin compositions and their role in antioxidant activities. Food Research International. 2012;46:250–259.
8. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2:1231–1246.
9. Prior RL. (2003): Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr: 78: 570-578.
10. Youdim K, Shukitt-Hale B, MacKinnon S, Kalt W, Joseph JA. Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo. Biochimica et Biophysica Acta (BBA)-General Subjects. 2000;1523(1):117–122.
11. Folmer F, Basavaraju U, Jaspars M, Hold G, El-Omar E, Dicato M. et al. Anticancer effects of bioactive berry compounds. Phytochemistry Reviews. 2014;13:295–322.
12. Akgül A. Tıbbi Araştırmalarda İstatistiksel Analiz Teknikleri. Yükseköğretim Kurulu Matbaası, Ankara (in Turkish), 1997.
13. Basu A, Lyons TJ. Strawberries, blueberries, and cranberries in the metabolic syndrome: Clinical perspectives. Journal Of Agricultural And Food Chemistry.2012;60: 5687–5692.
14. Nair AR, Elks CM, Vila J, Del Piero F, Paulsen DB, Francis J. A blueberry-enriched diet improves renal function and reduces oxidative stress in metabolic syndrome animals: Potential mechanism of TLR4-MAPK signaling pathway. PLoS One. 2014;9-11:e111976.
15. Wiseman W, Egan JM, Slemmer JE, Shaughnessy KS, Ballem K, Gottschall-Pass KT, Sweeney MI. Feeding blueberry diets inhibits angiotensin II-converting enzyme (ACE) activity in spontaneously hypertensive stroke-prone rats. Canadian Journal Of Physiology And Pharmacology. 2010;89:67–71.
16. Morazzoni P, Livio S, Scilingo A, Malandrino S. Vaccinium myrtillus anthocyanosides pharmacokinetics in rats. Arzneimittelforschung. 1991;41:128–131.
17. Sharp PE. and Villano J. The Laboratory Rat. In: The Laboratory Animal Pocket reference Series, Second Edition - CRC Press Book, 2013;399 P.
18. Funke I, Melzig MF. Effect of different phenolic compounds on alpha-amylase activity: Screening by microplate-reader based kinetic assay. Pharmazie, 2005;60:796–797.
19. Shaughnessy KS., Boswall IA., Scanlan AP., Gottschall-Pass KT., Sweeney MI. Diets containing blueberry extract lower blood pressure in spontaneously hypertensive stroke-prone rats. Nutrition Research. 2009;29:130–138.
20. Oh JS, Kim H, Vijayakumar A, Kwon O, Kim Y, Chang N. Association of dietary flavonoid ıntake with prevalence of type 2 diabetes mellitus and cardiovascular disease risk factors in korean women aged ≥ 30 years. Journal Of Nutritional Science And Vitaminology. 2017;63:51–58.
21. Bao L, Yao XS, Yau CC, Tsi D, Chia CS, Nagai H. et al. Protective effects of bilberry (Vaccinium myrtillus l.) extract on restraint stress-ınduced liver damage in mice. Journal Of Agricultural And Food Chemistry. 2008;56:7803–7807.
22. Rodriguez-Mateos A, Rendeiro C, Bergillos-Meca T, Tabatabaee S, George TW, Heiss C. et al. Intake and time dependence of blueberry flavonoid-induced improvements in vascular function: A randomized, controlled, double-blind, crossover intervention study with mechanistic insights into biological activity. The American Journal Of Clinical Nutrition. 2013;98:1179–1191.
23. Riso P, Klimis-Zacas D, Bo’ C, Martini D, Campolo J, Vendrame S. et al. Effect of a wild blueberry (Vaccinium angustifolium) drink intervention on markers of oxidative stress, inflammation and endothelial function in humans with cardiovascular risk factors. European Journal Of Nutrition. 2013;52:949-961.
24. Hertog MGL, Feskens EJM, Kromhout D, Hertog MGL, Hollman PCH, Hertog MGL. et al. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet. 1993;342:1007–1011.
25. Peterson JJ, Dwyer JT, Jacques PF, McCulloug ML. Associations between flavonoids and cardiovascular disease incidence or mortality in European and US populations. Nutrition Reviews. 2012;70:491–508.
26. Wright B, Spencer JPE, Lovegrove JA, Gibbins JM. Insights into dietary flavonoids as molecular templates for the design of anti-platelet drugs. Cardiovascular Research. 2013;97:13–22.
27. Golalipour MJ, Roshandel D, Roshandel G, Ghafari S, Kalavi M, Kalavi K. et al. Effect of lead intoxication and d-penicillamine treatment on hematological indices in rats. International Journal of Morphology. 2007;25:717–722.
28. Kalea AZ, Lamari FN, Theocharis AD, Cordopatis P, Schuschke DA, Karamanos NK, et al. Wild blueberry (Vaccinium angustifolium) consumption affects the composition and structure of glycosaminoglycans in Sprague-Dawley rat aorta. The Journal Of Nutritional Biochemistry. 2006;17:109–116.
29. Cao G, Shukitt-Hale B, Bickford PC, Joseph JA, McEwen J, Prior RL. Hyperoxia-induced changes in antioxidant capacity and the effect of dietary antioxidants. Journal of Applied Physiology. 1999;86:1817–1822.
30. Ponzo V, Goitre I, Fadda M, Gambino R, De Francesco A, Soldati L, et al. Dietary flavonoid intake and cardiovascular risk: a population-based cohort study. Journal Of Translational Medicine. 2015;13:218.
31. Alqurashi RM, Galante LA, Rowland IR, Spencer JPE, Commane DM. Consumption of a flavonoid-rich acai meal is associated with acute improvements in vascular function and a reduction in total oxidative status in healthy overweight men. The American Journal Of Clinical Nutrition. 2016;104:1227–1235.
32. Akbulut N. Akut Miyokard İnfarktüslü Hastalarda Ortalama Trombosit Hacmi ve Trombosit Sayısının Değerlendirilmesi., TC. Sağlık Bakanlığı Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi 2. İç Hastalıkları Kliniği Uzmanlık Tezi (unpublished), İstanbul, 2006.

VACCİNİUM MYRTİLLUS (BLUEBERRY) EKSTRAKTI VERİLEN RATLARDA BAZI HEMATOLOJİK VE BİYOKİMYASAL PARAMETRELERİN ARAŞTIRILMASI

Yıl 2018, Cilt: 2 Sayı: 2, 65 - 72, 02.05.2018

Dide Kılıçalp Kılınç Seda Nur Aksu Serdal Öğüt

Öz

Amaç: Bu çalışmada, Vaccinium myritillus (yaban mersini) ekstraktı ile beslenen ratların hematolojik ve biyokimyasal parametrelerinde meydana gelen değişiklikler araştırıldı.

Yöntem: Araştırmada hayvan materyali olarak rat kullanıldı. Ratlar, her grupta 10 adet olacak şekilde 2 ayrı gruba ayrıldı. Kontrol grubundaki ratlar ad libitum beslenmeye tabi tutuldu. Deneme gurubuna ise 8 hafta süreyle yaban mersini ekstraktı 20 mg/kg oranında içme sularına karıştırılarak verildi. Biyokimyasal parametrelerden albumin (Alb), alkalen fosfataz (ALP), alanin aminotransferaz (ALT), amilaz (AMY), kan üre nitrojen (BUN), kalsiyum (Ca⁺⁺), kreatinin (CRE), potasyum (K⁺), sodyum (Na⁺), fosfor (Phos), total bilurubin (TBIL), total protein (TP), açlık kan şekeri (FG), total kolesterol (TK); hematolojik parametreler olan WBC, LYM, MON, NEU, LY, MO, NE, RBC, HGB, HCT, MCV, MCH, MCHC, RDW PLT, PCT, MPV, PDW değerlerine bakıldı.

Bulgular: Deney süreci tamamlanıp, kan örnekleri alındıktan sonra yukarıdaki parametreler arasından; Kreatinin: 0.48’den 0.41’e düştü. MPV: 6.48’den 6.30’a, PDWc: 33.40’ dan 32.48 düzeyine düştü. Bu parametrelerin dışında kalan parametrelerde anlamlı bir değişiklik gözlenmedi.

Sonuç: Yaban mersininin kan parametreleri üzerinde olumsuz bir etkisinin olmadığı, aksine MPV ve PDW değerlerindeki anlamlı düşüş nedeniyle damar sertliği için bir risk faktörü olan oksidatif zarar sonucu parçalanmış trombositlerin atardamar çeperlerine yapışmasını ve arteroskloroz riskini de azaltabileceği düşünülebilir.

Anahtar Kelimeler

Biyokimyasal belirteçler, kan testleri, rat

Kaynakça

1. Ercişli S. Chemical composition of fruits in some rose (Rosa spp.) species. Food Chemistry. 2007;104(4):1379-1384.
2. Srividya AR, Nagasamy V, Vishnuvarthan VJ. Nutraceuticals as medicine: a review. Pharmanest. 2010;1(2):132–145.
3. Abuajah CI, Ogbonna AC, Osuji CM. Functional components and medicinal properties of food: a review. Journal Of Food Science And Technology. 2015;52:2522–2529.
4. Alvarez-Suarez JM, Giampieri F, Tulipan S, Casoli T, Stefano G Di, González-Paramás AM et al. One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans. The Journal Of Nutritional Biochemistry. 2014;25:289–294.
5. Wu X, Cao G, Prior RL. Absorption and metabolism of anthocyanins in elderly women after consumption of elderberry or blueberry. The Journal Of Nutrition. 2002;132:1865–1871.
6. Yi WG, Fischer J, Krewer G, Akoh CC. Phenolic compounds from blueberries can inhibit colon cancer cell proliferation and induce apoptosis. Journal Of Agricultural And Food Chemistry. 2005;53:7320–7329.
7. Li H, Deng Z, Zhu H, Hongyan Li, Zhu H, Hu C. et al. Highly pigmented vegetables: Anthocyanin compositions and their role in antioxidant activities. Food Research International. 2012;46:250–259.
8. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2:1231–1246.
9. Prior RL. (2003): Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr: 78: 570-578.
10. Youdim K, Shukitt-Hale B, MacKinnon S, Kalt W, Joseph JA. Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo. Biochimica et Biophysica Acta (BBA)-General Subjects. 2000;1523(1):117–122.
11. Folmer F, Basavaraju U, Jaspars M, Hold G, El-Omar E, Dicato M. et al. Anticancer effects of bioactive berry compounds. Phytochemistry Reviews. 2014;13:295–322.
12. Akgül A. Tıbbi Araştırmalarda İstatistiksel Analiz Teknikleri. Yükseköğretim Kurulu Matbaası, Ankara (in Turkish), 1997.
13. Basu A, Lyons TJ. Strawberries, blueberries, and cranberries in the metabolic syndrome: Clinical perspectives. Journal Of Agricultural And Food Chemistry.2012;60: 5687–5692.
14. Nair AR, Elks CM, Vila J, Del Piero F, Paulsen DB, Francis J. A blueberry-enriched diet improves renal function and reduces oxidative stress in metabolic syndrome animals: Potential mechanism of TLR4-MAPK signaling pathway. PLoS One. 2014;9-11:e111976.
15. Wiseman W, Egan JM, Slemmer JE, Shaughnessy KS, Ballem K, Gottschall-Pass KT, Sweeney MI. Feeding blueberry diets inhibits angiotensin II-converting enzyme (ACE) activity in spontaneously hypertensive stroke-prone rats. Canadian Journal Of Physiology And Pharmacology. 2010;89:67–71.
16. Morazzoni P, Livio S, Scilingo A, Malandrino S. Vaccinium myrtillus anthocyanosides pharmacokinetics in rats. Arzneimittelforschung. 1991;41:128–131.
17. Sharp PE. and Villano J. The Laboratory Rat. In: The Laboratory Animal Pocket reference Series, Second Edition - CRC Press Book, 2013;399 P.
18. Funke I, Melzig MF. Effect of different phenolic compounds on alpha-amylase activity: Screening by microplate-reader based kinetic assay. Pharmazie, 2005;60:796–797.
19. Shaughnessy KS., Boswall IA., Scanlan AP., Gottschall-Pass KT., Sweeney MI. Diets containing blueberry extract lower blood pressure in spontaneously hypertensive stroke-prone rats. Nutrition Research. 2009;29:130–138.
20. Oh JS, Kim H, Vijayakumar A, Kwon O, Kim Y, Chang N. Association of dietary flavonoid ıntake with prevalence of type 2 diabetes mellitus and cardiovascular disease risk factors in korean women aged ≥ 30 years. Journal Of Nutritional Science And Vitaminology. 2017;63:51–58.
21. Bao L, Yao XS, Yau CC, Tsi D, Chia CS, Nagai H. et al. Protective effects of bilberry (Vaccinium myrtillus l.) extract on restraint stress-ınduced liver damage in mice. Journal Of Agricultural And Food Chemistry. 2008;56:7803–7807.
22. Rodriguez-Mateos A, Rendeiro C, Bergillos-Meca T, Tabatabaee S, George TW, Heiss C. et al. Intake and time dependence of blueberry flavonoid-induced improvements in vascular function: A randomized, controlled, double-blind, crossover intervention study with mechanistic insights into biological activity. The American Journal Of Clinical Nutrition. 2013;98:1179–1191.
23. Riso P, Klimis-Zacas D, Bo’ C, Martini D, Campolo J, Vendrame S. et al. Effect of a wild blueberry (Vaccinium angustifolium) drink intervention on markers of oxidative stress, inflammation and endothelial function in humans with cardiovascular risk factors. European Journal Of Nutrition. 2013;52:949-961.
24. Hertog MGL, Feskens EJM, Kromhout D, Hertog MGL, Hollman PCH, Hertog MGL. et al. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet. 1993;342:1007–1011.
25. Peterson JJ, Dwyer JT, Jacques PF, McCulloug ML. Associations between flavonoids and cardiovascular disease incidence or mortality in European and US populations. Nutrition Reviews. 2012;70:491–508.
26. Wright B, Spencer JPE, Lovegrove JA, Gibbins JM. Insights into dietary flavonoids as molecular templates for the design of anti-platelet drugs. Cardiovascular Research. 2013;97:13–22.
27. Golalipour MJ, Roshandel D, Roshandel G, Ghafari S, Kalavi M, Kalavi K. et al. Effect of lead intoxication and d-penicillamine treatment on hematological indices in rats. International Journal of Morphology. 2007;25:717–722.
28. Kalea AZ, Lamari FN, Theocharis AD, Cordopatis P, Schuschke DA, Karamanos NK, et al. Wild blueberry (Vaccinium angustifolium) consumption affects the composition and structure of glycosaminoglycans in Sprague-Dawley rat aorta. The Journal Of Nutritional Biochemistry. 2006;17:109–116.
29. Cao G, Shukitt-Hale B, Bickford PC, Joseph JA, McEwen J, Prior RL. Hyperoxia-induced changes in antioxidant capacity and the effect of dietary antioxidants. Journal of Applied Physiology. 1999;86:1817–1822.
30. Ponzo V, Goitre I, Fadda M, Gambino R, De Francesco A, Soldati L, et al. Dietary flavonoid intake and cardiovascular risk: a population-based cohort study. Journal Of Translational Medicine. 2015;13:218.
31. Alqurashi RM, Galante LA, Rowland IR, Spencer JPE, Commane DM. Consumption of a flavonoid-rich acai meal is associated with acute improvements in vascular function and a reduction in total oxidative status in healthy overweight men. The American Journal Of Clinical Nutrition. 2016;104:1227–1235.
32. Akbulut N. Akut Miyokard İnfarktüslü Hastalarda Ortalama Trombosit Hacmi ve Trombosit Sayısının Değerlendirilmesi., TC. Sağlık Bakanlığı Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi 2. İç Hastalıkları Kliniği Uzmanlık Tezi (unpublished), İstanbul, 2006.

Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Sağlık Kurumları Yönetimi
Bölüm	Makaleler
Yazarlar	Dide Kılıçalp Kılınç Seda Nur Aksu Bu kişi benim Serdal Öğüt
Yayımlanma Tarihi	2 Mayıs 2018
Yayımlandığı Sayı	Yıl 2018 Cilt: 2 Sayı: 2

Kaynak Göster

APA	Kılıçalp Kılınç, D., Aksu, S. N., & Öğüt, S. (2018). INVESTIGATION OF SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RATS WERE GIVEN VACCINIUM MYRTILLUS (BLUEBERRY) EXTRACT. Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 2(2), 65-72.

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