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Isırgan (Urtica dioica L.), Keçiboynuzu (Ceratonia siliqua L.) ve Hayıt (Vitex agnus-castus L.) Bitkilerinin Caenorhabditis elegans' ta Fertiliteye Etkisi

Year 2024, Volume: 27 Issue: 4, 758 - 766

Handan Saraç Hülya Özpınar

https://doi.org/10.18016/ksutarimdoga.vi.1357294

Abstract

Bu çalışma, halk arasında geleneksel olarak infertilite tedavisinde kullanıldığı bilinen Isırgan (Urtica dioica L.), Keçiboynuzu (Ceratonia siliqua L.) ve Hayıt (Vitex agnus-castus L.) bitkilerinin su ekstraktının farklı konsantrasyonlarının, bir model organizma olan Caenorhabditis elegans (C. elegans)' ta fertilite üzerine olan etkilerini belirlemek amacıyla planlanmıştır. Çalışma kapsamında; bitkilerin su ekstraktlarının %0.1, %0.05, %0.02 ve %0.01’lik konsantrasyonları C. elegans standart besiyerine uygulanmış ve Koelle protokolüne göre 3 gün boyunca yumurta sayımı yapılmıştır. Ayrıca, her yumurta sayımı yapıldıktan bir gün sonra çatlamayan yumurtalar tespit edilerek, yumurta verimlilikleri de hesaplanmıştır. Analizler üç tekrarlı olarak yapılmıştır ve ortalamalar belirlenerek SPSS programı ile gruplar arasındaki farklar istatistiksel olarak karşılaştırılmıştır. Çalışmadan elde edilen sonuçlara göre, Isırgan, Keçiboynuzu ve Hayıt bitkilerinin fertilite üzerinde olumlu etkilere sahip olduğu belirlenmiştir. Bitkiler arasında fertiliteye en fazla etki eden bitkinin Isıgan olduğu bunu sırasıyla Hayıt ve Keçiboynuzu bitkilerinin takip ettiği belirlenmiştir. Ayrıca, doz uygulamalarında her üç bitkide de benzer şekilde en iyi etki en yüksek doz olan %0.1’ lik uygulamada tespit edilmiştir.

Keywords

Urtica dioica L. Ceratonia siliqua L. Vitex agnus-castus L. Caenorhabditis elegans Fertilite

Supporting Institution

Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri (CÜBAP)

Project Number

SMYO-028

References

  • Beşen, Ö. G. M. A. & Beji, N. K. (2014). Fertilite ve bitkiler. Androloji Bülteni 59, 286-289.
  • Bora, K. S. & Sharma, A. (2010). Phytochemical and pharmacological potential of Artemisia absinthium Linn. and Artemisia asiatica Nakai: a review. J Pharm Res. 3(2), 325-328.
  • Brassesco, M. E., Brandao, T. R., Silva, C. L., & Pintado, M. (2021). Carob bean (Ceratonia siliqua L.): A new perspective for functional food. Trends in Food Science & Technology 114, 310-322.
  • Corsi, A. K., Wightman, B., & Chalfie, M. (2015). A transparent window into biology: a primer on Caenorhabditis elegans. Genetics 200(2), 387-407.
  • Çetin, M. (2012). Hayvan beslemede antibiyotik ve antiparaziterlere alternatif olarak bitkisel ekstraklar ve Pelinotu'nun (Artemisia Absinthium) Kullanılması. KSÜ Doğa Bilimleri Dergisi 15(4), 58-63.
  • Daştan, T. & Saraç, H. (2018). Determination of the nutritional element concentrations of the Evelik plant (Rumex crispus L.). Cumhuriyet Science Journal 39(4),1020-1024.https://doi.org/10.17776/ csj.455688
  • Edirne, T., Arica, S. G., Gucuk, S., Yildizhan, R., Kolusari, A., Adali, E. & Can, M. (2010). Use of complementary and alternative medicines by a sample of Turkish women for infertility enhancement: a descriptive study. BMC complementary and alternative medicine 10, 1-7. http://www.biomedcentral.com/1472-6882/10/11.
  • Esposito, S., Bianco, A., Russo, R., Di Maro, A., Isernia, C., & Pedone, P. V. (2019). Therapeutic perspectives of molecules from Urtica dioica extracts for cancer treatment. Molecules 24(15), 2753.
  • Ezer, N. & Avcı, K. (2004). Çerkeş (Çankırı) yöresinde kullanılan halk ilaçları. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi 24(2), 67-80.
  • Fugh-Berman, A. & Kronenberg, F. (2003). Complementary and alternative medicine (CAM) in reproductive-age women: a review of randomized controlled trials. Reproductive Toxicology 17(2), 137-152. https://doi.org/10.1016/S0890-6238(02) 00128-4.
  • Gaware, V.M., Parjane, S.K., Merekar Abhijit, N., Pattan, S. R. & Dighe, N. S. (2009). Female infertility and its treatment by alternative medicine: a review. Journal of Chemical and Pharmaceutical Research 1(1), 148-162.
  • Hertweck, M., Hoppe, T. & Baumeister, R. (2003). C. elegans, a model for aging with high-throughput capacity. Experimental gerontology 38(3), 345-346. https://doi.org/10.1016/S0531-5565(02)00208-5.
  • Hossein-Rashidi, B. & Nemati, M. (2017). Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). Journal of Herbal Medicine 10, 24-30. https://doi.org/10.1016/j.hermed.2017.10.003.
  • İpekoğlu, H. Y. & Oral, H. B. (2019). İnfertilitede kullanilan geleneksel tedavi yöntemleri ve çeşitli uygulamalar: Isparta bölgesi. Motif Akademi Halkbilimi Dergisi 12(25), 105-119. https://doi.org/ 10.12981/mahder.523760.
  • Jalili, C., Salahshoor, M. R. & Naseri, A. (2014). Protective effect of Urtica dioica L against nicotine-induced damage on sperm parameters, testosterone, and testis tissue in mice. Iranian journal of reproductive medicine 12(6), 401-408.
  • Koelle, M. (2005). Quantitation of constitutive egg-laying. Yale University, New Haven, USA.
  • Niroumand, M. C., Heydarpour, F., & Farzaei, M. H. (2018). Pharmacological and therapeutic effects of Vitex agnus-castus L.: A review. Pharmacognosy Reviews 12 (23).
  • Olsen, A., Vantipalli, M. C. & Lithgow, G. J. (2006). Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatments. Biogerontology 7, 221-230. DOI: 10.1007/s10522-006-9018-x.
  • Özpınar, N. (2020). A model Organism for Antihelminthic Activity: Caenorhabditis elegans and Nigella sativa. Türkiye Parazitolojii Dergisi 44(1), 31. DOI:10.4274/tpd.galenos.2020.6759.
  • Özpınar, H. & Yüksek, M. (2019). Stevia rebaudiana Bertoni bitkisinin yaprak ekstraktının Caenorhabditis elegans bireylerinde yumurtadan çıkma ve fiziksel büyüme üzerine etkisi. International Journal of Academic Medicine and Pharmacy 1, 1-4.
  • Ozpinar, H., Ozpinar, N., & Karakus, S. (2017). The effects of Erzincan grape (Vitis vinifera spp., Cimin) and benzothiazol on a Caenorhabditis elegans organism model. Pharmacognosy Magazine 13(Suppl 2), S380.
  • Özpınar, H.,Özpınar, N., & Karakus, S. (2017). The Physiological and Genotoxic Effects of Water and Ethanol Extracts of Goji Berry (Lycium barbarum L.) on Model Organisms. Journal of Clinical and Experimental Investigations 8(3), 76-84.
  • Porta-de-la-Riva, M., Fontrodona, L., Villanueva, A. & Cerón, J. (2012). Basic Caenorhabditis elegans methods: synchronization and observation. JoVE (Journal of Visualized Experiments) (64), 1-9. DOI: 10.3791/4019.
  • Sanagoo, S., Farshbaf-Khalili, A., Asgharian, P., Hazhir, S. & Oskouei, B. S. (2021). Comparison of the effect of Ceratonia siliqua L. fruit oral capsule and vitamin E on semen parameters in men with idiopathic infertility: a triple-blind randomized controlled clinical trial. Journal of Complementary and Integrative Medicine 18(4), 791-796. https://doi.org/10.1515/jcim-2020-0095.
  • Saraç, H., Daştan, T., Demirbaş, A., Durna Daştan, S., Karaköy, T. & Durukan, H. (2018). Madımak (Polygonum cognatum Meissn.) bitki özütlerinin besin elementleri ve in vitro antikanserojen aktiviteleri yönünden değerlendirilmesi. Ziraat Fakültesi Dergisi 340-347.
  • Savaş, N. 2018. Avokado (Persea americana) ve Oğulotu (Melissa officinalis) Fonksiyonel Besinlerinin, Caenorhabditis elegans Termotoleransı Üzerine Etkilerinin İncelenmesi. [Master's thesis, Aydın Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü Beslenme ve Diyetetik Anabilim Dalı]. http://hdl.handle.net/ 11607/3505.
  • Springer, W. (2005). Caenorhabditis elegans as an experimental model organism to study Parkinson's disease-related genes: functional analysis of Parkin and α-synuclein [PhD Thesis, München, Univ., Diss. ].
  • Tan, L., Tong, Y., Sze, S. C. W., Xu, M., Shi, Y., Song, X. Y. & Zhang, T. T. (2012). Chinese herbal medicine for infertility with anovulation: a systematic review. The Journal of Alternative and Complementary Medicine 18(12), 1087-1100. https://doi.org/10.1089/acm.2011.0371.
  • Telefo, P. B., Lienou, L. L., Yemele, M. D., Lemfack, M. C., Mouokeu, C., Goka, C. S., ... & Moundipa, F. P. (2011). Ethnopharmacological survey of plants used for the treatment of female infertility in Baham, Cameroon. Journal of Ethnopharmacology 136(1), 178-187. https://doi.org/10.1016/j.jep.2011.04.036.
  • Ugulu, I., Baslar, S., Yorek, N. & Dogan, Y. (2009). The investigation and quantitative ethnobotanical evaluation of medicinal plants used around Izmir province, Turkey. Journal of Medicinal Plants Research 3(5), 345-367. http://www.academic journals.org/JMPR.
  • Ünlü, E. & Erdem, C. (2010). Deri yaşlanmasında korunma ve tedavi yöntemleri. Dermatoz 1(1), 23-31.
  • Vafaei, A., Mohammadi, S., Fazel, A., Soukhtanloo, M., Mohammadipour, A. & Beheshti, F. (2018). Effects of Carob (Ceratonia siliqua) on sperm quality, testicular structure, testosterone level, and oxidative stress in busulfan-induced infertile mice. Pharmaceutical Sciences 24(2), 104-111. doi: 10.15171/PS.2018.16.
  • Yılmaz, E. (2019). Türkiye’de Halk Arasında Yaygın Olarak Kullanılan Isırgan, Biberiye ve Keçiboynuzu Bitkilerinin İn vitro Koşullarda Sperm Fonksiyonu Parametrelerine Etkisi. [Master's thesis, Biruni Üniversitesi, Sağlık Bilimleri Enstitüsü]. https://hdl.handle.net/ 20.500.12445/1017.

The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans

Year 2024, Volume: 27 Issue: 4, 758 - 766

Handan Saraç Hülya Özpınar

https://doi.org/10.18016/ksutarimdoga.vi.1357294

Abstract

This study was carried out to determine the effects of different concentrations of water extract of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) plants, which are traditionally used in the treatment of infertility among the people, on fertility in Caenorhabditis elegans (C. elegans), which is a model organism. Scope of work; 0.1%, 0.05%, 0.02%, and 0.01% concentrations of the water extracts of the plants were applied to the C. elegans standard medium and egg counts were made for 3 days according to the Koelle protocol. In addition, one day after each egg count, uncracked eggs were determined, and egg productivity was calculated. The analyses were performed in triplicate, the averages were determined and the differences between the groups were statistically compared with the SPSS program. According to the results obtained from the study, it was determined that Nettle, Carob, and Chaste plants have positive effects on fertility. Among the plants, it was determined that the plant that affected fertility the most was Nettle, followed by Chaste and Carob plants, respectively. In addition, in terms of dosage applications, the best effect in all three plants has been observed at the highest dose of 0.1%.

Keywords

Urtica dioica L. Ceratonia siliqua L. Vitex agnus-castus L. Caenorhabditis elegans Fertility

Project Number

SMYO-028

References

  • Beşen, Ö. G. M. A. & Beji, N. K. (2014). Fertilite ve bitkiler. Androloji Bülteni 59, 286-289.
  • Bora, K. S. & Sharma, A. (2010). Phytochemical and pharmacological potential of Artemisia absinthium Linn. and Artemisia asiatica Nakai: a review. J Pharm Res. 3(2), 325-328.
  • Brassesco, M. E., Brandao, T. R., Silva, C. L., & Pintado, M. (2021). Carob bean (Ceratonia siliqua L.): A new perspective for functional food. Trends in Food Science & Technology 114, 310-322.
  • Corsi, A. K., Wightman, B., & Chalfie, M. (2015). A transparent window into biology: a primer on Caenorhabditis elegans. Genetics 200(2), 387-407.
  • Çetin, M. (2012). Hayvan beslemede antibiyotik ve antiparaziterlere alternatif olarak bitkisel ekstraklar ve Pelinotu'nun (Artemisia Absinthium) Kullanılması. KSÜ Doğa Bilimleri Dergisi 15(4), 58-63.
  • Daştan, T. & Saraç, H. (2018). Determination of the nutritional element concentrations of the Evelik plant (Rumex crispus L.). Cumhuriyet Science Journal 39(4),1020-1024.https://doi.org/10.17776/ csj.455688
  • Edirne, T., Arica, S. G., Gucuk, S., Yildizhan, R., Kolusari, A., Adali, E. & Can, M. (2010). Use of complementary and alternative medicines by a sample of Turkish women for infertility enhancement: a descriptive study. BMC complementary and alternative medicine 10, 1-7. http://www.biomedcentral.com/1472-6882/10/11.
  • Esposito, S., Bianco, A., Russo, R., Di Maro, A., Isernia, C., & Pedone, P. V. (2019). Therapeutic perspectives of molecules from Urtica dioica extracts for cancer treatment. Molecules 24(15), 2753.
  • Ezer, N. & Avcı, K. (2004). Çerkeş (Çankırı) yöresinde kullanılan halk ilaçları. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi 24(2), 67-80.
  • Fugh-Berman, A. & Kronenberg, F. (2003). Complementary and alternative medicine (CAM) in reproductive-age women: a review of randomized controlled trials. Reproductive Toxicology 17(2), 137-152. https://doi.org/10.1016/S0890-6238(02) 00128-4.
  • Gaware, V.M., Parjane, S.K., Merekar Abhijit, N., Pattan, S. R. & Dighe, N. S. (2009). Female infertility and its treatment by alternative medicine: a review. Journal of Chemical and Pharmaceutical Research 1(1), 148-162.
  • Hertweck, M., Hoppe, T. & Baumeister, R. (2003). C. elegans, a model for aging with high-throughput capacity. Experimental gerontology 38(3), 345-346. https://doi.org/10.1016/S0531-5565(02)00208-5.
  • Hossein-Rashidi, B. & Nemati, M. (2017). Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). Journal of Herbal Medicine 10, 24-30. https://doi.org/10.1016/j.hermed.2017.10.003.
  • İpekoğlu, H. Y. & Oral, H. B. (2019). İnfertilitede kullanilan geleneksel tedavi yöntemleri ve çeşitli uygulamalar: Isparta bölgesi. Motif Akademi Halkbilimi Dergisi 12(25), 105-119. https://doi.org/ 10.12981/mahder.523760.
  • Jalili, C., Salahshoor, M. R. & Naseri, A. (2014). Protective effect of Urtica dioica L against nicotine-induced damage on sperm parameters, testosterone, and testis tissue in mice. Iranian journal of reproductive medicine 12(6), 401-408.
  • Koelle, M. (2005). Quantitation of constitutive egg-laying. Yale University, New Haven, USA.
  • Niroumand, M. C., Heydarpour, F., & Farzaei, M. H. (2018). Pharmacological and therapeutic effects of Vitex agnus-castus L.: A review. Pharmacognosy Reviews 12 (23).
  • Olsen, A., Vantipalli, M. C. & Lithgow, G. J. (2006). Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatments. Biogerontology 7, 221-230. DOI: 10.1007/s10522-006-9018-x.
  • Özpınar, N. (2020). A model Organism for Antihelminthic Activity: Caenorhabditis elegans and Nigella sativa. Türkiye Parazitolojii Dergisi 44(1), 31. DOI:10.4274/tpd.galenos.2020.6759.
  • Özpınar, H. & Yüksek, M. (2019). Stevia rebaudiana Bertoni bitkisinin yaprak ekstraktının Caenorhabditis elegans bireylerinde yumurtadan çıkma ve fiziksel büyüme üzerine etkisi. International Journal of Academic Medicine and Pharmacy 1, 1-4.
  • Ozpinar, H., Ozpinar, N., & Karakus, S. (2017). The effects of Erzincan grape (Vitis vinifera spp., Cimin) and benzothiazol on a Caenorhabditis elegans organism model. Pharmacognosy Magazine 13(Suppl 2), S380.
  • Özpınar, H.,Özpınar, N., & Karakus, S. (2017). The Physiological and Genotoxic Effects of Water and Ethanol Extracts of Goji Berry (Lycium barbarum L.) on Model Organisms. Journal of Clinical and Experimental Investigations 8(3), 76-84.
  • Porta-de-la-Riva, M., Fontrodona, L., Villanueva, A. & Cerón, J. (2012). Basic Caenorhabditis elegans methods: synchronization and observation. JoVE (Journal of Visualized Experiments) (64), 1-9. DOI: 10.3791/4019.
  • Sanagoo, S., Farshbaf-Khalili, A., Asgharian, P., Hazhir, S. & Oskouei, B. S. (2021). Comparison of the effect of Ceratonia siliqua L. fruit oral capsule and vitamin E on semen parameters in men with idiopathic infertility: a triple-blind randomized controlled clinical trial. Journal of Complementary and Integrative Medicine 18(4), 791-796. https://doi.org/10.1515/jcim-2020-0095.
  • Saraç, H., Daştan, T., Demirbaş, A., Durna Daştan, S., Karaköy, T. & Durukan, H. (2018). Madımak (Polygonum cognatum Meissn.) bitki özütlerinin besin elementleri ve in vitro antikanserojen aktiviteleri yönünden değerlendirilmesi. Ziraat Fakültesi Dergisi 340-347.
  • Savaş, N. 2018. Avokado (Persea americana) ve Oğulotu (Melissa officinalis) Fonksiyonel Besinlerinin, Caenorhabditis elegans Termotoleransı Üzerine Etkilerinin İncelenmesi. [Master's thesis, Aydın Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü Beslenme ve Diyetetik Anabilim Dalı]. http://hdl.handle.net/ 11607/3505.
  • Springer, W. (2005). Caenorhabditis elegans as an experimental model organism to study Parkinson's disease-related genes: functional analysis of Parkin and α-synuclein [PhD Thesis, München, Univ., Diss. ].
  • Tan, L., Tong, Y., Sze, S. C. W., Xu, M., Shi, Y., Song, X. Y. & Zhang, T. T. (2012). Chinese herbal medicine for infertility with anovulation: a systematic review. The Journal of Alternative and Complementary Medicine 18(12), 1087-1100. https://doi.org/10.1089/acm.2011.0371.
  • Telefo, P. B., Lienou, L. L., Yemele, M. D., Lemfack, M. C., Mouokeu, C., Goka, C. S., ... & Moundipa, F. P. (2011). Ethnopharmacological survey of plants used for the treatment of female infertility in Baham, Cameroon. Journal of Ethnopharmacology 136(1), 178-187. https://doi.org/10.1016/j.jep.2011.04.036.
  • Ugulu, I., Baslar, S., Yorek, N. & Dogan, Y. (2009). The investigation and quantitative ethnobotanical evaluation of medicinal plants used around Izmir province, Turkey. Journal of Medicinal Plants Research 3(5), 345-367. http://www.academic journals.org/JMPR.
  • Ünlü, E. & Erdem, C. (2010). Deri yaşlanmasında korunma ve tedavi yöntemleri. Dermatoz 1(1), 23-31.
  • Vafaei, A., Mohammadi, S., Fazel, A., Soukhtanloo, M., Mohammadipour, A. & Beheshti, F. (2018). Effects of Carob (Ceratonia siliqua) on sperm quality, testicular structure, testosterone level, and oxidative stress in busulfan-induced infertile mice. Pharmaceutical Sciences 24(2), 104-111. doi: 10.15171/PS.2018.16.
  • Yılmaz, E. (2019). Türkiye’de Halk Arasında Yaygın Olarak Kullanılan Isırgan, Biberiye ve Keçiboynuzu Bitkilerinin İn vitro Koşullarda Sperm Fonksiyonu Parametrelerine Etkisi. [Master's thesis, Biruni Üniversitesi, Sağlık Bilimleri Enstitüsü]. https://hdl.handle.net/ 20.500.12445/1017.
There are 33 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Botany
Journal Section RESEARCH ARTICLE
Authors

Handan Saraç 0000-0001-7481-7978

Hülya Özpınar 0000-0001-8154-0874

Project Number SMYO-028
Early Pub Date April 23, 2024
Publication Date
Submission Date September 13, 2023
Acceptance Date January 5, 2024
Published in Issue Year 2024Volume: 27 Issue: 4

Cite

APA Saraç, H., & Özpınar, H. (2024). The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 758-766. https://doi.org/10.18016/ksutarimdoga.vi.1357294
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