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Nazilli-143 Pamuk (Gossypium hirsutum L.) Genotipinde Farklı Bitki Büyüme Düzenleyicilerinin Hipokotil Eksplantlarından Kallus İndüksiyonu Üzerine Etkisi ve Somatik Embriyo Aracılığıyla Bitkicik Rejenerasyonu

Year 2018, Volume: 28 Issue: 2, 55 - 61, 22.10.2018

Sadiye Hayta-smedley Nedim Özbek Arif Ansız Meltem Bayraktar Aynur Gurel

Abstract

ÖZ: Gossypium hirsutum L. cv.
Nazilli-143’ün hücre süspansiyon kültürlerinden somatik embriyo yolu ile
bitkicik rejenerasyonu için basit ve güvenilir bir protokol geliştirilmiştir.
Embriyogenik kallus 7 günlük fideciklerin hipokotil dokularından
başlatılmıştır. En yüksek embriyogenik kallus oranı Murashige ve Skoog (MS)
besin ortamının makro ve mikro elementleri, Gamborg (B5) besin ortamının
vitaminleri ile 30 g L-1 glukoz, 0,75 g L-1 MgCl2,
0,1 mg L-1 Kinetin ve 0,1 mg L-1 2,4 Diklorofenoksi asetik asit (2,4-D) içeren
ve % 0,7 (w/v) agar ile yarı katı hale getirilmiş besin ortamında (pH 5.8) elde
edilmiştir. Somatik embriyo indüksiyonu için süspansiyon kültürlerini kurmak
amacıyla, embriyogenik kalluslar bitki büyüme düzenleyicisi içermeyen sıvı MS
besin ortamında kültüre alınmıştır. Süspansiyon kültürleri süzülmüş ve globular
yapıdaki somatik embriyolar toplanarak çeşitli yarı katı besin ortamlarına
aktarılmışlardır. Embriyo çoğaltımı ve olgunlaşması en iyi, 1,3 g L-1
KNO3 eklenmiş, bitki büyüme düzenleyicisi içermeyen 2/3 MS besin
ortamında gözlenmiştir. Olgunlaşmış embriyoların % 36’sından bitkiciğe dönüşüm
gerçekleşmiştir. Köklü ve gerçek yapraklara sahip bitkicikler steril kültürden
uzaklaştırılmış ve bitki büyüme kabinine transfer edilmişlerdir.

Keywords

Somatik embriyogenez bitkicik rejenerasyonu süspansiyon kültürü pamuk Gossypium hirsutum L.

References

  • Aydin, Y., Z. Ipekci, T. Talas-Ogras, A. Zehir, A, K. Bayrovic, and N. Gozukirmizi. 2004 High frequency somatic embryogenesis in cotton. Biol. Plantarum 48 (4): 491-495.
  • Davidonis, G. H., and R. H. Hamilton. 1983. Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Science Letters 32: 89-93.
  • Finer, J. J. 1988. Plant regeneration from somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). Plant Cell Rep. 7: 481-494.
  • Firoozabady, E., and D. L. Deboer. 1993. Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). In Vitro Cell. Dev. Plant 299: 166-173.
  • Gamborg, O. L., R. A. Miller, and K. Ojima. 1968. Nutrition requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-159. Ganesan, M., R. Chandrasekar, B. D. Ranjitha Kumari, and N. Jayabalan. 2007. Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture. Biol. Plantarum 51 (3): 414-420. Juturu, V. N., G. K. Mekala, and P. B. Kirti 2015. Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.). Plant Cell Tissue and Organ Cult. 120: 813-839.
  • Khan, T., V. S. Reddy, and S. Leelavathi. 2010. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation. Plant Cell Tissue Organ Cult. 101: 323-330.
  • Kumria, R., V. G. Sunnichan, D. K. Das, S. K. Gupta, V. S. Reddy, R. K. Bhatnagar, and S. Leelavathi. 2003a. High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress. Plant Cell Rep. 21: 635-639. Kumria, R., S. Leelavathi, R. K. Bhatnagar, and V. S. Reddy. 2003b. Regeneration and genetic transformation of cotton: Present status and future perspectives. Plant Tissue Culture 13: 211-225. Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15: 473-497.
  • Price, H. J., and R. H. Smith. 1979. Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss. Planta 145: 305-307.
  • Shoemaker, R.C., L. J. Couche, and D. W. Galbraith. 1986. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. 3: 178-181.
  • Trolinder, N. L. and J. R. Goodin. 1988a. Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime. Plant Cell Tissue Organ Cult. 12: 178-181.
  • Trolinder, N. L., and J. R. Goodin. 1988b. Somatic embryogenesis in cotton (Gossypium) II. Requirements for embryo development and plant regeneration. Plant Cell Tissue Organ Cult. 12: 43-53.
  • Wang, Y. X., X. F. Wang, Z. Y. Ma, G. Y. Zhang, and G. Y. Han. 2006. Somatic embryogenesis and plant regeneration from two recalcitrant genotypes of Gossypium hirsutum L. Agricultural Sciences in China 5 (5): 323-329.
  • Zhang, B. H., F. Liu, and C. B. Yak. 2000. Plant regeneration via somatic embryogenesis in cotton. Plant Cell Tissue Organ Cult. 60: 89-94.

The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143

Year 2018, Volume: 28 Issue: 2, 55 - 61, 22.10.2018

Sadiye Hayta-smedley Nedim Özbek Arif Ansız Meltem Bayraktar Aynur Gurel

Abstract

Keywords

Somatic embryogenesis plantlet regeneration suspension culture cotton Gossypium hirsutum L.

References

  • Aydin, Y., Z. Ipekci, T. Talas-Ogras, A. Zehir, A, K. Bayrovic, and N. Gozukirmizi. 2004 High frequency somatic embryogenesis in cotton. Biol. Plantarum 48 (4): 491-495.
  • Davidonis, G. H., and R. H. Hamilton. 1983. Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Science Letters 32: 89-93.
  • Finer, J. J. 1988. Plant regeneration from somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). Plant Cell Rep. 7: 481-494.
  • Firoozabady, E., and D. L. Deboer. 1993. Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). In Vitro Cell. Dev. Plant 299: 166-173.
  • Gamborg, O. L., R. A. Miller, and K. Ojima. 1968. Nutrition requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-159. Ganesan, M., R. Chandrasekar, B. D. Ranjitha Kumari, and N. Jayabalan. 2007. Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture. Biol. Plantarum 51 (3): 414-420. Juturu, V. N., G. K. Mekala, and P. B. Kirti 2015. Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.). Plant Cell Tissue and Organ Cult. 120: 813-839.
  • Khan, T., V. S. Reddy, and S. Leelavathi. 2010. High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation. Plant Cell Tissue Organ Cult. 101: 323-330.
  • Kumria, R., V. G. Sunnichan, D. K. Das, S. K. Gupta, V. S. Reddy, R. K. Bhatnagar, and S. Leelavathi. 2003a. High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress. Plant Cell Rep. 21: 635-639. Kumria, R., S. Leelavathi, R. K. Bhatnagar, and V. S. Reddy. 2003b. Regeneration and genetic transformation of cotton: Present status and future perspectives. Plant Tissue Culture 13: 211-225. Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15: 473-497.
  • Price, H. J., and R. H. Smith. 1979. Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss. Planta 145: 305-307.
  • Shoemaker, R.C., L. J. Couche, and D. W. Galbraith. 1986. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. 3: 178-181.
  • Trolinder, N. L. and J. R. Goodin. 1988a. Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime. Plant Cell Tissue Organ Cult. 12: 178-181.
  • Trolinder, N. L., and J. R. Goodin. 1988b. Somatic embryogenesis in cotton (Gossypium) II. Requirements for embryo development and plant regeneration. Plant Cell Tissue Organ Cult. 12: 43-53.
  • Wang, Y. X., X. F. Wang, Z. Y. Ma, G. Y. Zhang, and G. Y. Han. 2006. Somatic embryogenesis and plant regeneration from two recalcitrant genotypes of Gossypium hirsutum L. Agricultural Sciences in China 5 (5): 323-329.
  • Zhang, B. H., F. Liu, and C. B. Yak. 2000. Plant regeneration via somatic embryogenesis in cotton. Plant Cell Tissue Organ Cult. 60: 89-94.
There are 13 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Sadiye Hayta-smedley This is me

Nedim Özbek This is me

Arif Ansız This is me

Meltem Bayraktar This is me

Aynur Gurel

Publication Date October 22, 2018
Submission Date June 8, 2017
Published in Issue Year 2018 Volume: 28 Issue: 2

Cite

APA Hayta-smedley, S., Özbek, N., Ansız, A., Bayraktar, M., et al. (2018). The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 28(2), 55-61.
AMA Hayta-smedley S, Özbek N, Ansız A, Bayraktar M, Gurel A. The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU. October 2018;28(2):55-61.
Chicago Hayta-smedley, Sadiye, Nedim Özbek, Arif Ansız, Meltem Bayraktar, and Aynur Gurel. “The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium Hirsutum L.) Genotype Nazilli-143”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28, no. 2 (October 2018): 55-61.
EndNote Hayta-smedley S, Özbek N, Ansız A, Bayraktar M, Gurel A (October 1, 2018) The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28 2 55–61.
IEEE S. Hayta-smedley, N. Özbek, A. Ansız, M. Bayraktar, and A. Gurel, “The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143”, ANADOLU, vol. 28, no. 2, pp. 55–61, 2018.
ISNAD Hayta-smedley, Sadiye et al. “The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium Hirsutum L.) Genotype Nazilli-143”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28/2 (October 2018), 55-61.
JAMA Hayta-smedley S, Özbek N, Ansız A, Bayraktar M, Gurel A. The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU. 2018;28:55–61.
MLA Hayta-smedley, Sadiye et al. “The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium Hirsutum L.) Genotype Nazilli-143”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, vol. 28, no. 2, 2018, pp. 55-61.
Vancouver Hayta-smedley S, Özbek N, Ansız A, Bayraktar M, Gurel A. The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU. 2018;28(2):55-61.
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